Globose to ovoid-ellipsoid, papillate sporangium
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P. agathidicida sporangium |
P. agathidicia oogonia P. agathidicida oogonia with SEM (top) and light microscopy (bottom) |
P. agathidicia oogonia Light micrograph of P. agathidicida oospore (Scale bar equals 15 µm) |
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Boot wash to station to control spread of P. agathidicida Use of hypochlorite solution applied through a “livestock drench-gun”, integrated with a soil grate to allow potentially contaminated soil to be collected |
P. agathidicida oospores in planta Oospores in the roots of kauri seedlings inoculated with P. agathidicida. The root has been cleared with potassium hydroxide and bleached with peroxide before being stained with trypan blue (scale bar =100 µm). |
P. agathidicia disease symptoms on kauri Crown decline of mature kauri, with branchlets with little or no leaves |
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P. agathadicida disease symptom Excavated lesion, with outer bark removed showing extent of disease-front |
P. agathidicia sporangia Differentiation of the cytoplasm within papillate sporangia into acid fuchsin stained zoospores |
Tāne Mahuta “Lord of the Forest” kauri tree Tāne Mahuta “Lord of the Forest” is a giant kauri tree (approximately 47 metres in height) in the Waipoua Forest of Northland Region, New Zealand. Its age is unknown but is estimated to be between 1,250 and 2,500 years |
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P. agathidicida oospores Oospores of P. agathidicida in the roots of kauri seedlings inoculated with P. agathidicida. The root has been cleared with potassium hydroxide and bleached with peroxide, before being stained with Trypan Blue |
P. agathidicida growth on CMA Diffuse, non-patterned, colony morphology of ICMP 16471 (the original “Gadgil isolate”) after 10-days incubation at 20°C in the dark |
Comparative gametangial morphology of Phytophthora Clade 5 species Comparative gametangial morphology of Phytophthora Clade 5 species, with SEM (top) and light microscopy (bottom). P. heveae has smooth walled oogonia with funnel-shaped, amphigynous antheridia. P. agathidicida has mildly stipulate oogonia with globose amphigynous antheridia. P.cocois has mildly bullate oogonia with reflexed amphigynous antheridia. P. castaneae has coarsely bullate oogonium with rugose protuberances and narrow amphigynous antheridia (Weir et al. 2015). |


