02281nas a2200229 4500008004100000022001400041245012000055210006900175260001200244300001200256490000800268520147800276100001401754700002501768700002301793700002101816700002701837700001901864700001801883700002501901856012501926 2013 eng d a0031-949X00aMembrane-based oligonucleotide array developed from multiple markers for the detection of many Phytophthora species0 aMembranebased oligonucleotide array developed from multiple mark c01/2013 a43 - 540 v1033 a

Most Phytophthora spp. are destructive plant pathogens; therefore, effective monitoring and accurate early detection are important means of preventing potential epidemics and outbreaks of diseases. In the current study, a membrane-based oligonucleotide array was developed that can detect Phytophthora spp. reliably using three DNA regions; namely, the internal transcribed spacer (ITS), the 5′ end of cytochrome c oxidase 1 gene (cox1), and the intergenic region between cytochrome c oxidase 2 gene (cox2) and cox1 (cox2-1 spacer). Each sequence data set contained ≈250 sequences representing 98 described and 15 undescribed species of Phytophthora. The array was validated with 143 pure cultures and 35 field samples. Together, nonrejected oligonucleotides from all three markers have the ability to reliably detect 82 described and 8 undescribed Phytophthora spp., including several quarantine or regulated pathogens such as Phytophthora ramorum. Our results showed that a DNA array containing signature oligonucleotides designed from multiple genomic regions provided robustness and redundancy for the detection and differentiation of closely related taxon groups. This array has the potential to be used as a routine diagnostic tool for Phytophthora spp. from complex environmental samples without the need for extensive growth of cultures.

1 aChen, Wen1 aDjama, Zeinab Robleh1 aCoffey, Michael, D1 aMartin, Frank, N1 aBilodeau, Guillaume, J1 aRadmer, Lorien1 aDenton, Geoff1 aLévesque, André, C uhttps://forestphytophthora.org/references/membrane-based-oligonucleotide-array-developed-multiple-markers-detection-many