02502nas a2200205 4500008004100000245013100041210006900172260001200241300001400253520178100267100001502048700001202063700001602075700001502091700001602106700001902122700001302141700001602154856012602170 2013 eng d00aIdentification of Phytophthora species baited and isolated from forest soil and streams in northwestern Yunnan province, China0 aIdentification of Phytophthora species baited and isolated from  c01/2013 an/a - n/a3 a

Phytophthora species were surveyed by collecting soil samples and placing bait leaves in selected streams during June–October in the years 2005, 2006 and 2010 at three sites in oak forests in Diqing Tibetan Autonomous Prefecture of NW Yunnan province, China. Seventy-three isolates of Phytophthora spp. were recovered from 135 baited leaf samples and 81 soil samples. Eight Phytophthora species were identified by observation of morphological features and ITS1-5.8S-ITS2 rDNA sequence analysis. The eight taxa included two well-known species P. gonapodyides and P. cryptogea, two recently described species P. gregata and P. plurivora, two named but as yet undescribed taxa, P. taxon PgChlamydo and P. taxon Salixsoil, and two previously unrecognized species, Phytophthora sp.1 and P. sp.2. The most numerous species, P. taxon PgChlamydo, and the second most abundant species, P. taxon Salixsoil, were recovered at all three sites. Phytophthora cryptogea was detected only once at site Nixi. Phytophthora gregata and P. sp.2 were isolated from a stream only at site Bitahai, while the other three species were each found at two sites. Phylogenetic analysis revealed that the isolates belonged to three ITS clades, one species including six isolates in clade 2, six species including 66 isolates in clade 6 and one species in clade 8. There was a relatively rich species and genetic diversity of Phytophthora detected in the investigated regions where the forest biotic and abiotic factors affecting the growth and evolution of Phytophthora populations were diverse.

1 aHuai, W -x1 aTian, G1 aHansen, E M1 aZhao, W -x1 aGoheen, E M1 aGrünwald, N J1 aCheng, C1 aBelbahri, L uhttps://forestphytophthora.org/references/identification-phytophthora-species-baited-and-isolated-forest-soil-and-streams