01624nas a2200181   4500008004100000022001300041245012800054210006900182300001400251490000700265520092800272653002601200653001901226653003001245100001601275700001601291856013501307       2001                        eng d  a1437478100aMolecular diagnosis of Phytophthora lateralis in trees, water, and foliage baits using multiplex polymerase chain reaction.0 aMolecular diagnosis of Phytophthora lateralis in trees water and  a275 - 2830 v313 a<p>A polymerase chain reaction (PCR)-based protocol for detection of <em>Phytophthora lateralis</em> in plant tissues and water is described. Base-pair (bp) deletions in both of the ribosomal DNA internal transcribed spacer (ITS) regions in <em>P. lateralis</em> were used to design complementary PCR primer sequences that amplify a 738 bp fragment only if <em>P. lateralis</em> DNA is present in the sample. Universal control primers based on conserved sequences of the nuclear ribosomal small subunit are included in a multiplexed reaction, providing an internal check on the procedure. The universal primers amplify an approximately 550 bp fragment that is common to plants, protists, and true fungi. The procedure reliably detects <em>P. lateralis</em> in cedar stem tissues and in roots. Positive reactions were obtained with as few as 200 <em>P. lateralis</em> zoospores in water. (English) [ABSTRACT FROM AUTHOR]</p>10aPHYTOPHTHORA diseases10aPLANT diseases10apolymerase chain reaction1 aWinton, L M1 aHansen, E M  uhttps://forestphytophthora.org/references/molecular-diagnosis-phytophthora-lateralis-trees-water-and-foliage-baits-using-multiplex